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resource source identifier antibodies rabbit monoclonal anti sox9 abcam ![Expression of <t>SOX9</t> in lower urogenital tract and prostatic buds of the male mouse fetus. (A, left panel) IHC showing SOX9 expression in a sagittal section of the lower urogenital tract at E15.0. Brown staining is seen throughout epithelium of the bladder (BL), Wolffian ducts (WD), UGS and urethra (UR), and in patches of UGS mesenchyme [40x]. (A, right panel) SOX9 expression in the UGS is visualized at higher magnification [100x]. (B, left panel) SOX9 expression is shown for two anterior buds in a transverse section of UGS at E18.5 [40x] and (B, right panel) in a single anterior bud (AB) at E18.5 [200x]. Nuclei were counterstained blue with hematoxylin. Other abbreviations: anterior (A), dorsal (D) and ventral (V) regions of the UGS. Images are representative of n=3 litter independent fetuses, where each fetus came from a different litter.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7329/pmc06927329/pmc06927329__nihms-1543025-f0001.jpg) Resource Source Identifier Antibodies Rabbit Monoclonal Anti Sox9 Abcam, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/resource source identifier antibodies rabbit monoclonal anti sox9 abcam/product/Abcam Average 99 stars, based on 1 article reviews
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Image Search Results
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Expression of SOX9 in lower urogenital tract and prostatic buds of the male mouse fetus. (A, left panel) IHC showing SOX9 expression in a sagittal section of the lower urogenital tract at E15.0. Brown staining is seen throughout epithelium of the bladder (BL), Wolffian ducts (WD), UGS and urethra (UR), and in patches of UGS mesenchyme [40x]. (A, right panel) SOX9 expression in the UGS is visualized at higher magnification [100x]. (B, left panel) SOX9 expression is shown for two anterior buds in a transverse section of UGS at E18.5 [40x] and (B, right panel) in a single anterior bud (AB) at E18.5 [200x]. Nuclei were counterstained blue with hematoxylin. Other abbreviations: anterior (A), dorsal (D) and ventral (V) regions of the UGS. Images are representative of n=3 litter independent fetuses, where each fetus came from a different litter.
Article Snippet: Reagent or
Techniques: Expressing, Staining
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Conditional knockout of Sox9 in UGE and confirmation of loss of SOX9 expression in lower urinary tract epithelium. Conditional knockout of Sox9 (cKO Sox9) in the UGE was mediated by Shh driven cre recombinase expression. (A) Lower urinary tract of an E14.5 cre Reporter male fetus stained with Bluo-gal. Blue staining indirectly shows cre recombinase expression restricted to epithelium of the bladder, UGS, and urethra (20x). (B and C) IHC showing SOX9 expression (brown) in sagittal sections of the lower urogenital tract and testis (inset, bottom right) from a representative control (B) and Sox9 cKO (C) fetus at E16.5 (40x magnification; nuclei counterstained blue with hematoxylin). The UGS is identified by a red, rectangular box. In the control fetus (B), SOX9 expression is seen throughout epithelium of the lower urogenital tract. In the Sox9 cKO fetus (C) SOX 9 expression was completely absent from epithelium of the bladder (BL), UGS, and urethra (UR) prior to prostatic budding. Specificity of the conditional Sox9 knockout is demonstrated by normal SOX9 expression in Wolffian ducts (WD), mesenchymal patch (arrow), and Sertoli cells of the testis in Sox9 cKO fetuses. All images are representative of n = 3 litter-independent male fetuses.
Article Snippet: Reagent or
Techniques: Knock-Out, Expressing, Staining
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Study design.
Article Snippet: Reagent or
Techniques: Staining
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: WM IHC for cadherin-1 (CDH1) stained epithelium in control male UGS (A) and Sox9 cKO male UGS (B). Sox9 is knocked out specifically in the UGE, before prostatic budding initiation begins. The representative control UGS (A) and Sox9 cKO UGS (B) were harvested at P0.5 from mouse neonates from the same litter. For each UGS, the right panel is an enlargement of the UGS (boxed area) in the left panel (20x). All bud types in the Sox9 cKO UGS failed to elongate. From this view (right panels), failed elongation of buds in the Sox9 cKO UGS (B) is most easily observed for anterior buds (AB, pseudocolored blue) and ventral buds (VB, pseudocolored green). Abbreviations: bladder and bladder neck (BL), seminal vesicle (SV), urethra (UR), dorsolateral buds (DLB) and urogenital sinus (UGS).
Article Snippet: Reagent or
Techniques: Staining
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Gene ontology analysis of male Sox9 cKO UGE transcriptome predicts decreased UGE cell migration.
Article Snippet: Reagent or
Techniques: Migration, Chemotaxis Assay
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Union (light tan) of the Venn diagram shows 31 genes in the male UGE at E16.75 that have Sox9-dependent expression (tan, control male vs Sox9 cKO male) in common with sex-dependent expression (white, control male vs control female). Also 18 of these 31 genes are candidates for promoting bud formation and 3 are candidates for inhibiting bud formation (Table 3). Results for males are based on n = 6 litter independent, male UGEs for both control and Sox9 cKO groups, respectively, and results for females are based on n = 5 litter independent, female UGEs for both control and Sox9 cKO groups.
Article Snippet: Reagent or
Techniques: Expressing
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Putative regulators of bud development.
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Techniques:
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Confirmation of Sox9-dependent genes in male mouse UGE by PCR and microarray. Comparison of microarray analysis to qRT-PCR in determining relative expression of Sox9-dependent transcripts in the UGE of control (Con) vs Sox9 cKO male mouse fetuses at E16.75. Genes selected for this comparison were initially identified by microarray analysis as being Sox9-dependent by comparing their relative transcript abundance between control male vs Sox9 cKO UGEs at E16.75. Some of these Sox9-dependent genes were then discovered by gene ontology analysis to function in cell migration or other functions while other Sox9-dependent genes in the male were identified as putative prostatic bud promoters and inhibitors. Sox9-dependent genes, within these functional groupings, were selected for confirmation of differential expression by qRT-PCR. UGEs were assessed by microarray (n = 6, both groups) and qRT-PCR (n = 4, both groups). UGEs analyzed by qRT-PCR were separate and distinct from those analyzed by microarray. The expression of these transcripts in control samples, determined by microarray from the bi-weight average signal, has been set to 1.0, while the expression in Sox9 cKO samples is shown as the fold-change of Sox9 cKO signal, to control signal. For each transcript analyzed with qRT-PCR, the average expression relative to Actb1 (2−ΔCt) in control UGEs have been set to 1.0. Expression of these transcripts relative to Actb1 in Sox9 cKO samples is shown as the fold-change of the relative expression seen in controls (2−ΔΔCt). For both methods error bars represent SEM. Asterisks indicate a significant difference between control and Sox9 cKO (p ≤ 0.05) when the same method of gene expression analysis is used; ns indicates no significant difference (p > 0.05). Abbreviations: conditional knockout (cKO), urogenital epithelium (UGE), standard error of the mean (SEM) and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).
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Techniques: Microarray, Quantitative RT-PCR, Expressing, Migration, Functional Assay, Knock-Out, Polymerase Chain Reaction
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Union (dark tan) of the Venn diagram shows that 16 genes exhibit Sox9-dependent expression in both male and female UGEs at E16.75. The number of Sox9-dependent genes that are significantly upregulated and downregulated (± 1.3 fold, p ≤ 0.05) are given for male UGEs (tan) and female UGEs (red) based on a comparison of gene expression (control vs Sox9 cKO) within each sex. For each sex, the number of differentially regulated, annotated transcripts is shown at the top, and unannotated transcripts in parentheses at the bottom. Names of the 16 Sox9-dependent genes, common to both sexes (union, dark tan), are given in Table 4. Results for males are based on n = 6 litter independent, male UGEs for both control and Sox9 cKO groups, respectively. Findings for females are based on n = 5 litter independent, female UGEs for both control and Sox9 cKO groups.
Article Snippet: Reagent or
Techniques: Expressing
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: SOX9 dependent genes differentially expressed, in common, in male and female UGEs at E16.75.
Article Snippet: Reagent or
Techniques:
![Heatmap of 151 differentially expressed, Sox9-dependent genes are shown for the “Male” and “Female” mouse UGE at E16.75 (Supplementary Table 8). Fold change in expression of individual genes in the “Male” [Sox9 cKO male vs control male] and “Female” [Sox9 cKO female vs control female] UGE is depicted in the heatmap as yellow (upregulated) and blue (down-regulated). Of these 151 genes, 16 (11%) were significantly different from control in both sexes (regulated in common); 58 (38%) were significantly different from control only in the “Female” and 77 (51%) were different only in the “Male”. Results for males are based on n = 6 litter independent, male UGEs for both control and Sox9 cKO groups, respectively. Findings for females are based on n = 5 litter independent, female UGEs for both control and Sox9 cKO groups.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7329/pmc06927329/pmc06927329__nihms-1543025-f0007.jpg)
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Heatmap of 151 differentially expressed, Sox9-dependent genes are shown for the “Male” and “Female” mouse UGE at E16.75 (Supplementary Table 8). Fold change in expression of individual genes in the “Male” [Sox9 cKO male vs control male] and “Female” [Sox9 cKO female vs control female] UGE is depicted in the heatmap as yellow (upregulated) and blue (down-regulated). Of these 151 genes, 16 (11%) were significantly different from control in both sexes (regulated in common); 58 (38%) were significantly different from control only in the “Female” and 77 (51%) were different only in the “Male”. Results for males are based on n = 6 litter independent, male UGEs for both control and Sox9 cKO groups, respectively. Findings for females are based on n = 5 litter independent, female UGEs for both control and Sox9 cKO groups.
Article Snippet: Reagent or
Techniques: Expressing
Journal: Gene expression patterns : GEP
Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
doi: 10.1016/j.gep.2019.119075
Figure Lengend Snippet: Key resources Table
Article Snippet: Reagent or
Techniques: Plasmid Preparation, Recombinant, Microarray, Software, Sequencing, Staining